Physiology of the conjugation process in the yeast Hansenula wingei.
نویسنده
چکیده
The yeast Hansenula wingei is a favourable organism for study of the physiology of the conjugation process (cell fusion). Microscopic observations on fusion are presented which reveal that the mating cells in contact fuse by a softening of the cell wall, followed by formation of a conjugation tube, dissolution of the cross-walls between them, and formation of a new bud a t the point of juncture of the two cells. A simple technique for studying fusion in a liquid medium is described. Up to 80 yo of the cells will fuse in 5 hr. at 30' in a medium containing an ener,gy source, MgSO, and potassium phosphate, under conditions in which no growth or budding of unmated cells would occur. Synthesis of new protein is required for fusion as shown by inhibition by amino acid analogues. The precursors for this new protein come from the amino acid pool. Both mating types must be able to function for conjugation to occur. It is postulated that each mating type produces an inducer which diffuses into the opposite type. Each inducer brings about the synthesis of a wall-softening enzyme which acts upon the cell producing it. Cell fusion is viewed as an extension of the normal budding process.
منابع مشابه
Association constant of the sex-specific agglutinin in the yeast, Hansenula wingei.
Association constants were determined for absorption of the sex-specific agglutinin from mating type 5 of Hansenula wingei by cells of type 21, the opposite mating type. Absorption was determined by following the radiolabeled sulfur in purified agglutinin extracted from cells grown in 35S-labeled medium. Association was proved to be reversible under conditions where cell agglutination was In se...
متن کاملSterols as Components in the Mating Reaction of Hansenula Wingei.
Hunt, D. E. (Charles V. Chapin Hospital, Providence, R.I.) and P. L. Carpenter. Sterols as components in the mating reaction of Hansenula wingei. J. Bacteriol. 86:845-847. 1963.-Agglutinative mating strains 5 and 21 of the yeast Hansenula wingei did not agglutinate after the cells of these two strains were treated with nystatin. The agglutination-inhibiting action of this antibiotic could not b...
متن کاملBiochemical and cellular changes occuring during conjugation in Hansenula wingei.
Brock, Thomas D. (Indiana University, Bloomington). Biochemical and cellular changes occurring during conjugation in Hansenula wingei. J. Bacteriol. 90:1019-1025. 1954.-A technique has been devised for deagglutinating mixed populations of conjugating cells so as to be able to visualize microscopically early stages of the conjugation process. A cell can form a conjugation tube only when in conta...
متن کاملAmino Acids as Protective Compounds in Ultraviolet-irradiated Hansenula Wingei.
Hunt, D. E. (Charles V. Chapin Hospital, Providence, R.I.). Amino acids as protective compounds in ultraviolet-irradiated Hansenula wingei. J. Bacteriol. 88:893-895. 1964.-"Prefeeding" Hansenula wingei with small concentrations of either tryptophan, cystine, tyrosine, phenylalanine, leucine, cysteine, threonine, isoleucine, ethionine, norvaline, or histidine protected this yeast against inactiv...
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ورودعنوان ژورنال:
- Journal of general microbiology
دوره 26 شماره
صفحات -
تاریخ انتشار 1961